Amazon Prime customers in the US will now have to pay $12.99 (£9.37) a month for the service after the tech giant bumped up prices from $10.99.
Shares in the company opened up more than one per cent.
Students members will also face a monthly price rise of one dollar to $6.49.
But users who choose to pay up-front annual fees for the service will still pay $99 for standard membership and $49 for students.
Customers get free two-day delivery and discounted next-day options.
In the UK, this caused problems in the run-up to Christmas as some customers complained deliveries had been delayed.